control for the entire RT-PCR experiment as the reagents for this sample were provided in the RevertAid kit. The No Template Control (NTC) reactions contained all reagents for the RT-PCR reaction, except nuclease-free water was added in place of RNA template. Gel electrophoresis: The RT-PCR reactions were electrophoresed on a 1% agaorse gelAuthor: Kevin Gardner, Ian Lorang, Stefanie Otto-Hitt. High-throughput multiplex PCR genotyping for 35 red blood cell antigens in blood donors C. Jungbauer, 1C. M. Hobel, D. W. M. Schwartz2 & W. R. Mayr1,2 1Vienna Blood Centre, Blood Service, Austrian Red Cross, Vienna, Austria 2Department of Blood Group Serology and Transfusion Medicine, Medical University of Vienna, Vienna, Austria Received: 10 February PCR reactions were amplified in a standard thermocycler using the following program: 94C for 2 minutes followed by 45 cycles of 94C for 30 seconds, annealing temperature for 30 seconds and 68C for 30 seconds. PCR reactions were then gel purified by running on a 1% polyacrylamide gel in 1X TAE buffer or directly used for subsequent sdgcoolstore.com by: 3.
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